Why compromise any aspect of performance in your cloning experiments? DNA ligases from NEB set the industry standard for function and purity, and are available at exceptional value.

• Choose T4 DNA Ligase to meet the demands of a variety of reaction conditions, including multi-insert ligations or large fragment ligation.
   
• Choose NEB's Quick Ligation Kits to experience extreme fast 5 min reactions at room temperature for all routine ligations.

With continous product development and extensive quality controls, DNA ligases from NEB ensure success in your ligation reactions.

Advantages:

• Highly pure enzyme with no lot-to-lot variation 
   

• Flexible reaction setup
  
   
• Active in a variety of reaction buffers
   

• Ligation within only 5 min using NEBs Quick Ligation Kit

 5 min
 Ligations:

 Choose the 
 NEB Quick Ligation Kit
 to experience extreme
 fast 5 min reactions at
 room temperature for
 all routine ligations.

 
Now with sample pack
 of „ready-to-use“
 NEB 5-alpha competent
 E. coli cells 
 free of charge!

T4 DNA Ligase

E.coli DNA Ligase

Taq DNA Ligase

9°N DNA Ligase

T4 RNA Ligase 1

T4 RNA Ligase 2

T4 RNA
Ligase 2, truncated

T4 RNA
Ligase 2, truncated,
K227Q

recombinant

√

√

√

√

√

√

√

thermostable

√

√

recommended for DNA cloning

√

ligation of cohesive DNA ends

√

√

ligation of blunt DNA ends

√

Nicks in dsDNA

√

√

√

√

Nicks in dsRNA

√

Joining of RNA & DNA in a ds-structure

√

√

Labeling of 3' termini of RNA

√

√

√

Joining ssRNA

√

Ligase Detection Reaction
& Ligase Chain Reaction

√

√

Ligation of preadenylated adaptors to RNA (miRNA-Cloning)

√

√